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Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution
Although de novo computational enzyme design has been shown to be feasible, the field is still in its infancy: the kinetic parameters of designed enzymes are still orders of magnitude lower than those of naturally occurring ones. Nonetheless, designed enzymes can be improved by directed evolution, a...
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| Main Authors: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3889864/ https://ncbi.nlm.nih.gov/pubmed/21277311 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2011.01.041 |
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