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Quantitative Affinity Chromatography. Determination of Binding Constants by Elution with Competitive Inhibitors
The affinity chromatographic separation procedure was tested for its utility in quantitating the affinity of proteins to both insolubilized ligand and corresponding competing soluble ligand. An expression has been derived that (1) allows the determination of binding constants for the interaction of...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1974
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC388459/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4526212/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.6.2382 |
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