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Quantitative Affinity Chromatography. Determination of Binding Constants by Elution with Competitive Inhibitors

The affinity chromatographic separation procedure was tested for its utility in quantitating the affinity of proteins to both insolubilized ligand and corresponding competing soluble ligand. An expression has been derived that (1) allows the determination of binding constants for the interaction of...

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Библиографические подробности
Опубликовано в: :Proc Natl Acad Sci U S A
Главные авторы: Dunn, Ben M., Chaiken, Irwin M.
Формат: Artigo
Язык:Inglês
Опубликовано: National Academy of Sciences 1974
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Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC388459/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4526212/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.6.2382
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