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Quantitative Affinity Chromatography. Determination of Binding Constants by Elution with Competitive Inhibitors
The affinity chromatographic separation procedure was tested for its utility in quantitating the affinity of proteins to both insolubilized ligand and corresponding competing soluble ligand. An expression has been derived that (1) allows the determination of binding constants for the interaction of...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1974
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC388459/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4526212/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.6.2382 |
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