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Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases
RNA-guided endonucleases (RGENs), derived from the prokaryotic adaptive immune system known as CRISPR/Cas, enable targeted genome engineering in cells and organisms. RGENs are ribonucleoproteins that consist of guide RNA and Cas9, a protein component originated from Streptococcus pyogenes. These enz...
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| Autori principali: | , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Cold Spring Harbor Laboratory Press
2014
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3875854/ https://ncbi.nlm.nih.gov/pubmed/24253446 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.162339.113 |
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