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Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease

The type II CRISPR-associated protein Cas9 recognizes and cleaves target DNA with the help of two guide RNAs (gRNAs; tracrRNA and crRNA). However, the detailed mechanisms and kinetics of these gRNAs in the Cas9 nuclease activity are unclear. Here, we investigate the structural roles of gRNAs in the...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Nat Commun
Päätekijät: Lim, Youngbin, Bak, So Young, Sung, Keewon, Jeong, Euihwan, Lee, Seung Hwan, Kim, Jin-Soo, Bae, Sangsu, Kim, Seong Keun
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Nature Publishing Group 2016
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5097132/
https://ncbi.nlm.nih.gov/pubmed/27804953
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms13350
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