Selective Exo-Enzymatic Labeling (SEEL) of N-Glycans of Living Cells by Recombinant ST6Gal I()

SEEL CELL SURFACE GLYCANS: N-Glycans of living cells could be selectively tagged by exogenously administered recombinant ST6Gal I sialyltransferase and azido-modified CMP-Neu5Ac followed by a strain promoted cycloaddition using biotin modified dibenzylcyclooctynol (DIBO). The methodology will make i...

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Main Authors: Mbua, Ngalle Eric, Li, Xiuru, Flanagan-Steet, Heather R., Meng, Lu, Aoki, Kazuhiro, Moremen, Kelley W., Wolfert, Margreet A., Steet, Richard, Boons, Geert-Jan
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3869382/
https://ncbi.nlm.nih.gov/pubmed/24129959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201307095
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