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In vitro Reconstitution and Analysis of the 6-Deoxyerythronolide B Synthase

Notwithstanding an extensive literature on assembly line polyketide synthases such as the 6-deoxyerythronolide B synthase (DEBS), a complete naturally occurring synthase has never been reconstituted in vitro from purified protein components. Here, we describe the fully reconstituted DEBS and quantit...

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Detalhes bibliográficos
Main Authors: Lowry, Brian, Robbins, Thomas, Weng, Chih-Hisang, O'Brien, Robert V., Cane, David E., Khosla, Chaitan
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3858836/
https://ncbi.nlm.nih.gov/pubmed/24161212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja409048k
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