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Expression, purification, crystallization and preliminary X-ray diffraction analysis of the aspartate transcarbamoylase domain of human CAD

Aspartate transcarbamoylase (ATCase) catalyzes the synthesis of N-carbamoyl-l-aspartate from carbamoyl phosphate and aspartate in the second step of the de novo biosynthesis of pyrimidines. In prokaryotes, the first three activities of the pathway, namely carbamoyl phosphate synthetase (CPSase), ATC...

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Detalhes bibliográficos
Main Authors: Ruiz-Ramos, Alba, Lallous, Nada, Grande-García, Araceli, Ramón-Maiques, Santiago
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3855736/
https://ncbi.nlm.nih.gov/pubmed/24316846
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113031114
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