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Purification, crystallization and preliminary X-ray diffraction analysis of aspartate semialdehyde dehydrogenase (Rv3708c) from Mycobacterium tuberculosis

Aspartate semialdehyde dehydrogenase from Mycobacterium tuberculosis (Asd, ASADH, Rv3708c), which is the second enzyme in the lysine/homoserine-biosynthetic pathways, has been expressed heterologously in Escherichia coli. The enzyme was purified using affinity and gel-filtration chromatographic tech...

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Detalhes bibliográficos
Main Authors: Vyas, Rajan, Kumar, Vijay, Panjikar, Santosh, Karthikeyan, Subramanian, Kishan, K. V. Radha, Tewari, Rupinder, Weiss, Manfred S.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2374159/
https://ncbi.nlm.nih.gov/pubmed/18323599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108002753
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