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Expression, purification, crystallization and preliminary X-ray diffraction analysis of the dihydroorotase domain of human CAD
CAD is a 243 kDa eukaryotic multifunctional polypeptide that catalyzes the first three reactions of de novo pyrimidine biosynthesis: glutamine-dependent carbamyl phosphate synthetase, aspartate transcarbamylase and dihydroorotase (DHO). In prokaryotes, these activities are associated with monofuncti...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
International Union of Crystallography
2012
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3515377/ https://ncbi.nlm.nih.gov/pubmed/23143245 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112038857 |
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