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Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase

The subtilase SBT3 from Solanum lycopersicum (tomato) was purified from a tomato cell culture and crystallized using the sitting-drop vapour-diffusion method. A native data set was collected to 2.5 Å resolution at 100 K using synchrotron radiation. For experimental phasing, CsCl-derivative and tetra...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Rose, Rolf, Huttenlocher, Franziska, Cedzich, Anna, Kaiser, Markus, Schaller, Andreas, Ottmann, Christian
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2675601/
https://ncbi.nlm.nih.gov/pubmed/19407393
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109013979
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