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Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase
The subtilase SBT3 from Solanum lycopersicum (tomato) was purified from a tomato cell culture and crystallized using the sitting-drop vapour-diffusion method. A native data set was collected to 2.5 Å resolution at 100 K using synchrotron radiation. For experimental phasing, CsCl-derivative and tetra...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
International Union of Crystallography
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2675601/ https://ncbi.nlm.nih.gov/pubmed/19407393 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109013979 |
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