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Purification, crystallization and preliminary X-ray diffraction analysis of ThiM from Staphylococcus aureus

ThiM [5-(hydroxyethyl)-4-methylthiazole kinase; EC 2.7.1.50] from Staphylococcus aureus is an essential enzyme of thiamine or vitamin B(1) metabolism and has been crystallized by the vapour-diffusion method. The crystals belonged to the primitive space group P1, with unit-cell parameters a = 62.06,...

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Detalhes bibliográficos
Main Authors: Drebes, Julia, Perbandt, Markus, Wrenger, Carsten, Betzel, Christian
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3080155/
https://ncbi.nlm.nih.gov/pubmed/21505246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111004192
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