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Generating gene knockout rats by homologous recombination in embryonic stem cells
We describe here a detailed protocol for generating gene knockout rats by homologous recombination in embryonic stem (ES) cells. This protocol comprises the following procedures: derivation and expansion of rat ES cells, construction of gene-targeting vectors, generation of gene-targeted rat ES cell...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3855261/ https://ncbi.nlm.nih.gov/pubmed/21637202 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.338 |
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