Direct atomic force microscope imaging of EcoRI endonuclease site specifically bound to plasmid DNA molecules.

Direct imaging with the atomic force microscope has been used to identify specific nucleotide sequences in plasmid DNA molecules. This was accomplished using EcoRI (Gln-111), a mutant of the restriction enzyme that has a 1000-fold greater binding affinity than the wild-type enzyme but with cleavage...

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Bibliografiske detaljer
Main Authors: Allison, D P, Kerper, P S, Doktycz, M J, Spain, J A, Modrich, P, Larimer, F W, Thundat, T, Warmack, R J
Format: Artigo
Sprog:Inglês
Udgivet: 1996
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC38552/
https://ncbi.nlm.nih.gov/pubmed/8799111
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