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Direct atomic force microscope imaging of EcoRI endonuclease site specifically bound to plasmid DNA molecules.

Direct imaging with the atomic force microscope has been used to identify specific nucleotide sequences in plasmid DNA molecules. This was accomplished using EcoRI (Gln-111), a mutant of the restriction enzyme that has a 1000-fold greater binding affinity than the wild-type enzyme but with cleavage...

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Publicado no:Proc Natl Acad Sci U S A
Principais autores: Allison, D P, Kerper, P S, Doktycz, M J, Spain, J A, Modrich, P, Larimer, F W, Thundat, T, Warmack, R J
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1996
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC38552/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8799111/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.17.8826
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