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Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells

BACKGROUND: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human embryonic stem (hES) cells, differentiation i...

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Detalhes bibliográficos
Main Authors: Vossaert, Liesbeth, O’Leary, Thomas, Van Neste, Christophe, Heindryckx, Björn, Vandesompele, Jo, De Sutter, Petra, Deforce, Dieter
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3848990/
https://ncbi.nlm.nih.gov/pubmed/24028740
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-14-21
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