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Accurate RT-qPCR gene expression analysis on cell culture lysates

Gene expression quantification on cultured cells using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) typically involves an RNA purification step that limits sample processing throughput and precludes parallel analysis of large numbers of samples. An approach in which cDN...

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書目詳細資料
Main Authors: Van Peer, Gert, Mestdagh, Pieter, Vandesompele, Jo
格式: Artigo
語言:Inglês
出版: Nature Publishing Group 2012
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3257325/
https://ncbi.nlm.nih.gov/pubmed/22355736
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep00222
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