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Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples
Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms of reverse transcription polymerase chain rea...
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| Foilsithe in: | Sci Rep |
|---|---|
| Main Authors: | , , , , , |
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
Nature Publishing Group
2016
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4761903/ https://ncbi.nlm.nih.gov/pubmed/26898768 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep21418 |
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