Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples

Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms of reverse transcription polymerase chain rea...

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Detaylı Bibliyografya
Yayımlandı:Sci Rep
Asıl Yazarlar: Zeka, Fjoralba, Vanderheyden, Katrien, De Smet, Els, Cuvelier, Claude A., Mestdagh, Pieter, Vandesompele, Jo
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Nature Publishing Group 2016
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4761903/
https://ncbi.nlm.nih.gov/pubmed/26898768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep21418
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