Llwytho...
RNA-Seq analysis to capture the transcriptome landscape of a single cell
We describe here a protocol for digital transcriptome analysis in a single mouse blastomere using a deep sequencing approach. An individual blastomere was first isolated and put into lysate buffer by mouth pipette. Reverse transcription was then performed directly on the whole cell lysate. After thi...
Wedi'i Gadw mewn:
| Prif Awduron: | , , , , , , |
|---|---|
| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
2010
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| Pynciau: | |
| Mynediad Ar-lein: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3847604/ https://ncbi.nlm.nih.gov/pubmed/20203668 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.236 |
| Tagiau: |
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