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RNA-Seq analysis to capture the transcriptome landscape of a single cell
We describe here a protocol for digital transcriptome analysis in a single mouse blastomere using a deep sequencing approach. An individual blastomere was first isolated and put into lysate buffer by mouth pipette. Reverse transcription was then performed directly on the whole cell lysate. After thi...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3847604/ https://ncbi.nlm.nih.gov/pubmed/20203668 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.236 |
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