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Orthogonal Cas9 Proteins for RNA-Guided Gene Regulation and Editing

The Cas9 protein from the Streptococcus pyogenes CRISPR-Cas immune system has been adapted for both RNA-guided genome editing and gene regulation in a variety of organisms, but can mediate only a single activity at a time within any given cell. Here we characterize a set of fully orthogonal Cas9 pro...

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Main Authors: Esvelt, Kevin M., Mali, Prashant, Braff, Jonathan L., Moosburner, Mark, Yaung, Stephanie J., Church, George M.
格式: Artigo
語言:Inglês
出版: 2013
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3844869/
https://ncbi.nlm.nih.gov/pubmed/24076762
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2681
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