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Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach
We develop an in vivo library-on-library methodology to simultaneously assess single guide RNA (sgRNA) activity across ~1,400 genomic loci. Assaying across multiple human cell types, end-processing enzymes, and two Cas9 orthologs, we unravel underlying nucleotide sequence and epigenetic parameters....
Shranjeno v:
| izdano v: | Nat Methods |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2015
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5292764/ https://ncbi.nlm.nih.gov/pubmed/26167643 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3473 |
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