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Kinetic and Isotopic Characterization of L-Proline Dehydrogenase from Mycobacterium tuberculosis
The monofunctional proline dehydrogenase (ProDH) from Mycobacterium tuberculosis performs the flavin-dependent oxidation of L-proline to Δ(1)-pyrroline-5-carboxylate (P5C) in the proline catabolic pathway. The ProDH gene, prub, was cloned into the pYUB1062 vector, and the C-terminal His-tagged 37 kD...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3839249/ https://ncbi.nlm.nih.gov/pubmed/23834473 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi400338f |
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