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Determination of enzyme mechanisms by radiationless energy transfer kinetics
Rigorous definition of the elementary steps of an enzymatic reaction requires visualization of transient enzyme—substrate (ES) complexes. Measurement of radiationless energy transfer (RET) between enzyme tryptophan residues and a fluorescent dansyl (5-dimethylaminonaphthalene-1-sulfonyl) substrate p...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1979
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383672/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/288055/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.6.2684 |
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