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Surveyor Substrates: Energy-Transfer Gauges of Active Center Topography during Catalysis

An approach is described for the simultaneous measurement of catalysis, the distance from an active-site moiety to fluorescent substrate moieties of an enzyme substrate complex, and the microenvironment of such substrate groups. It is illustrated by means of a three-component energy transfer relay s...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Latt, S. A., Auld, D. S., Vallee, B. L.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1970
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC283363/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/5274465/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.67.3.1383
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