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Surveyor Substrates: Energy-Transfer Gauges of Active Center Topography during Catalysis

An approach is described for the simultaneous measurement of catalysis, the distance from an active-site moiety to fluorescent substrate moieties of an enzyme substrate complex, and the microenvironment of such substrate groups. It is illustrated by means of a three-component energy transfer relay s...

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Autors principals: Latt, S. A., Auld, D. S., Vallee, B. L.
Format: Artigo
Idioma:Inglês
Publicat: 1970
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC283363/
https://ncbi.nlm.nih.gov/pubmed/5274465
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