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Determination of enzyme mechanisms by radiationless energy transfer kinetics
Rigorous definition of the elementary steps of an enzymatic reaction requires visualization of transient enzyme—substrate (ES) complexes. Measurement of radiationless energy transfer (RET) between enzyme tryptophan residues and a fluorescent dansyl (5-dimethylaminonaphthalene-1-sulfonyl) substrate p...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1979
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383672/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/288055/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.6.2684 |
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