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Determination of enzyme mechanisms by radiationless energy transfer kinetics
Rigorous definition of the elementary steps of an enzymatic reaction requires visualization of transient enzyme—substrate (ES) complexes. Measurement of radiationless energy transfer (RET) between enzyme tryptophan residues and a fluorescent dansyl (5-dimethylaminonaphthalene-1-sulfonyl) substrate p...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1979
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC383672/ https://ncbi.nlm.nih.gov/pubmed/288055 |
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