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Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans

pRK212.2, a derivative of the broad host range plasmid RK2, contains two EcoRI cleavage fragments, A and B, neither of which can replicate by itself in Escherichia coli. Fragment A (41.7 kilobases), but not fragment B (14.4 kilobases), can be cloned by insertion into the unrelated plasmids mini-F an...

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Detalhes bibliográficos
Main Authors: Figurski, David H., Helinski, Donald R.
Formato: Artigo
Idioma:Inglês
Publicado em: 1979
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC383447/
https://ncbi.nlm.nih.gov/pubmed/377280
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