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Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers.

Mutated forms of trfA, the replication protein gene of plasmid RK2, that support a minimal RK2 origin plasmid in Escherichia coli at copy numbers up to 23-fold higher than normal have been isolated. Six such high-copy-number (copy-up) mutations were mapped and sequenced. In each case, a single base...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Durland, R H, Toukdarian, A, Fang, F, Helinski, D R
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1990
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC213367/
https://ncbi.nlm.nih.gov/pubmed/2193921
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