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Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers.

Mutated forms of trfA, the replication protein gene of plasmid RK2, that support a minimal RK2 origin plasmid in Escherichia coli at copy numbers up to 23-fold higher than normal have been isolated. Six such high-copy-number (copy-up) mutations were mapped and sequenced. In each case, a single base...

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Udgivet i:J Bacteriol
Principais autores: Durland, R H, Toukdarian, A, Fang, F, Helinski, D R
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1990
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213367/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2193921/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.7.3859-3867.1990
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