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Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans

pRK212.2, a derivative of the broad host range plasmid RK2, contains two EcoRI cleavage fragments, A and B, neither of which can replicate by itself in Escherichia coli. Fragment A (41.7 kilobases), but not fragment B (14.4 kilobases), can be cloned by insertion into the unrelated plasmids mini-F an...

詳細記述

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書誌詳細
出版年:Proc Natl Acad Sci U S A
主要な著者: Figurski, David H., Helinski, Donald R.
フォーマット: Artigo
言語:Inglês
出版事項: National Academy of Sciences 1979
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383447/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/377280/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.4.1648
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