Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans
pRK212.2, a derivative of the broad host range plasmid RK2, contains two EcoRI cleavage fragments, A and B, neither of which can replicate by itself in Escherichia coli. Fragment A (41.7 kilobases), but not fragment B (14.4 kilobases), can be cloned by insertion into the unrelated plasmids mini-F an...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1979
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383447/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/377280/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.4.1648 |
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