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Detection and accumulation of tetrahedral intermediates in elastase catalysis.

Tetrahedral intermediates in the reaction of elastase with specific di- and tripeptide p-nitroanilide substrates have been detected, accumulated, and stabilized at high pH by using subzero temperatures and fluid aqueous/organic cryosolvents. The tetrahedral adducts are characterized by spectra with...

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Autors principals: Fink, A L, Meehan, P
Format: Artigo
Idioma:Inglês
Publicat: 1979
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC383430/
https://ncbi.nlm.nih.gov/pubmed/36609
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