Detection and accumulation of tetrahedral intermediates in elastase catalysis.
Tetrahedral intermediates in the reaction of elastase with specific di- and tripeptide p-nitroanilide substrates have been detected, accumulated, and stabilized at high pH by using subzero temperatures and fluid aqueous/organic cryosolvents. The tetrahedral adducts are characterized by spectra with...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1979
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383430/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/36609/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.4.1566 |
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