Detection and accumulation of tetrahedral intermediates in elastase catalysis.

Tetrahedral intermediates in the reaction of elastase with specific di- and tripeptide p-nitroanilide substrates have been detected, accumulated, and stabilized at high pH by using subzero temperatures and fluid aqueous/organic cryosolvents. The tetrahedral adducts are characterized by spectra with...

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Argitaratua izan da:	Proc Natl Acad Sci U S A
Egile Nagusiak:	Fink, A L, Meehan, P
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	National Academy of Sciences 1979
Gaiak:	Research Article
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383430/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/36609/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.4.1566
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