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Detection and accumulation of tetrahedral intermediates in elastase catalysis.

Tetrahedral intermediates in the reaction of elastase with specific di- and tripeptide p-nitroanilide substrates have been detected, accumulated, and stabilized at high pH by using subzero temperatures and fluid aqueous/organic cryosolvents. The tetrahedral adducts are characterized by spectra with...

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Sábháilte in:
Sonraí bibleagrafaíochta
Foilsithe in:Proc Natl Acad Sci U S A
Príomhchruthaitheoirí: Fink, A L, Meehan, P
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: National Academy of Sciences 1979
Ábhair:
Rochtain ar líne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383430/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/36609/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.4.1566
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