Affinity grid-based cryo-EM of PKC binding to RACK1 on the ribosome

Affinity grids (AG) are specialized EM grids that bind macromolecular complexes containing tagged proteins to obtain maximum occupancy for structural analysis through single-particle EM. In this study, utilizing AG, we show that His-tagged activated PKC βII binds to the small ribosomal subunit (40S)...

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Bibliographic Details
Main Authors: Sharma, Gyanesh, Pallesen, Jesper, Das, Sanchaita, Grassucci, Robert, Langlois, Robert, Hampton, Cheri M., Kelly, Deborah F., des Georges, Amedee, Frank, Joachim
Format: Artigo
Language:Inglês
Published: 2012
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3833090/
https://ncbi.nlm.nih.gov/pubmed/23228487
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2012.11.006
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