Affinity grid-based cryo-EM of PKC binding to RACK1 on the ribosome

Affinity grids (AG) are specialized EM grids that bind macromolecular complexes containing tagged proteins to obtain maximum occupancy for structural analysis through single-particle EM. In this study, utilizing AG, we show that His-tagged activated PKC βII binds to the small ribosomal subunit (40S)...

Ful tanımlama

Kaydedildi:
Detaylı Bibliyografya
Asıl Yazarlar: Sharma, Gyanesh, Pallesen, Jesper, Das, Sanchaita, Grassucci, Robert, Langlois, Robert, Hampton, Cheri M., Kelly, Deborah F., des Georges, Amedee, Frank, Joachim
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2012
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3833090/
https://ncbi.nlm.nih.gov/pubmed/23228487
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2012.11.006
Etiketler: Etiketle
Etiket eklenmemiş, İlk siz ekleyin!

Benzer Materyaller