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Control of eIF-2 phosphatase activity in rabbit reticulocyte lysate.

A sensitive assay requiring picomole amounts of [32P]eIF-2 to measure eIF-2 phosphatase activity has been developed. Dephosphorylation of [32P]eIF-2 alpha (38,000-dalton subunit) is extremely rapid (t 1/2 = 20 sec) and occurs at the same rate in both hemin-supplemented and hemin-depleted lysates. In...

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Bibliografische gegevens
Hoofdauteurs: Safer, B, Jagus, R
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1979
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC383195/
https://ncbi.nlm.nih.gov/pubmed/220606
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