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Control of eIF-2 phosphatase activity in rabbit reticulocyte lysate.

A sensitive assay requiring picomole amounts of [32P]eIF-2 to measure eIF-2 phosphatase activity has been developed. Dephosphorylation of [32P]eIF-2 alpha (38,000-dalton subunit) is extremely rapid (t 1/2 = 20 sec) and occurs at the same rate in both hemin-supplemented and hemin-depleted lysates. In...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Proc Natl Acad Sci U S A
Prif Awduron: Safer, B, Jagus, R
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 1979
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383195/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/220606/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.3.1094
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