Control of eIF-2 phosphatase activity in rabbit reticulocyte lysate.
A sensitive assay requiring picomole amounts of [32P]eIF-2 to measure eIF-2 phosphatase activity has been developed. Dephosphorylation of [32P]eIF-2 alpha (38,000-dalton subunit) is extremely rapid (t 1/2 = 20 sec) and occurs at the same rate in both hemin-supplemented and hemin-depleted lysates. In...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1979
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383195/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/220606/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.3.1094 |
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