Expression in Escherichia coli of chemically synthesized genes for human insulin.

Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322. The cloned synthetic genes were then fused to an Escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. The insulin peptides were cleaved fr...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Goeddel, D V, Kleid, D G, Bolivar, F, Heyneker, H L, Yansura, D G, Crea, R, Hirose, T, Kraszewski, A, Itakura, K, Riggs, A D
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1979
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC382885/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/85300/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.1.106
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