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Expression in Escherichia coli of chemically synthesized genes for human insulin.
Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322. The cloned synthetic genes were then fused to an Escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. The insulin peptides were cleaved fr...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1979
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC382885/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/85300/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.1.106 |
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