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Site-Directed Mutagenesis of Catalytic Residues in N(5)-Carboxyaminoimidazole Ribonucleotide Synthetase

N(5)-CAIR synthetase, an essential enzyme in microorganisms, converts 5-aminoimidazole ribonucleotide (AIR) and bicarbonate to N(5)-CAIR with the aid of ATP. Previous X-ray crystallographic analyses of Aspergillus clavatus N(5)-CAIR synthetase postulated that R271, H273, and K353 were important for...

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Main Authors: Dewal, Mahender B., Firestine, Steven M.
Formato: Artigo
Idioma:Inglês
Publicado: 2013
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3820426/
https://ncbi.nlm.nih.gov/pubmed/23899325
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi400444y
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