Analysis of the role of cysteine residues in isopenicillin N synthetase activity by site-directed mutagenesis.
The predicted amino acid sequences of isopenicillin N synthetase from both Cephalosporium acremonium and Penicillium chrysogenum have two cysteine residues in analogous positions (Cys-106 and Cys-255 in the C. acremonium numbering). To examine the role of these cysteine residues in the activity of t...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1987
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC298931/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3112774/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.16.5705 |
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