High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherichia coli.

The pcbC gene, which encodes isopenicillin N synthase (IPNS), was subcloned from Streptomyces clavuligerus into Escherichia coli by using the pT7 series of plasmid vectors. The polymerase chain reaction was used to introduce an NdeI site at the translation initiation codon of pcbC, allowing the gene...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Durairaj, M, Doran, J L, Jensen, S E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1992
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183222/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1476443/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.12.4038-4041.1992
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