High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherichia coli.
The pcbC gene, which encodes isopenicillin N synthase (IPNS), was subcloned from Streptomyces clavuligerus into Escherichia coli by using the pT7 series of plasmid vectors. The polymerase chain reaction was used to introduce an NdeI site at the translation initiation codon of pcbC, allowing the gene...
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| Published in: | Appl Environ Microbiol |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1992
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183222/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1476443/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.12.4038-4041.1992 |
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