High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherichia coli.

The pcbC gene, which encodes isopenicillin N synthase (IPNS), was subcloned from Streptomyces clavuligerus into Escherichia coli by using the pT7 series of plasmid vectors. The polymerase chain reaction was used to introduce an NdeI site at the translation initiation codon of pcbC, allowing the gene...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Durairaj, M, Doran, J L, Jensen, S E
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1992
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183222/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1476443/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.12.4038-4041.1992
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